The Two-Hybrid System

نویسندگان

  • Carolina Vollert
  • Peter Uetz
چکیده

The bait and prey fusions are co-expressed in yeast where a physical interaction between X and Y leads to the reconstitution of a functional transcription factor that binds to the promoter of a reporter gene (Fig.1). Consequently, transcription of the reporter gene is switched on. A protein-protein interaction is therefore translated into reporter gene activity which can be detected or measured. The interaction must take place in the nucleus for the subsequent activation of the reporter gene. For this purpose non-nuclear proteins are targeted to the nucleus.

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تاریخ انتشار 2003